SELEKSI BAKTERI PENGHASIL ANTIBIOTIK DARI RHIZOSFER MANGROVE UNTUK PENGENDALIAN VIBRIOSIS PADA UDANG WINDU

Abstrak

Shrimp that live in natural habitats and in farming are often exposed to vibriosis disease caused by Vibrio infection. Currently the prevention of the disease in shrimp caused by pathogenic bacteria using synthetic antibiotics and other drugs. However, the use of antibiotics and drugs that are not wise has given rise to a new problem, namely the existence of pathogenic bacterial resistance to antibiotics, residues in shrimp body and reduce consumer confidence in the quality of the product. An alternative to prevention of disease in shrimp fishery commodities in particular is the use of natural antibioticproducing bacteria that can inhibit growth and kill pathogenic bacteria. Selection of antibiotic-producing bacteria from the mangrove rhizosphere based on their ability to inhibit Vibrio harveyi which is bacterium of vibriosis disease-causing. Selection of antibiotic-producing bacteria from the mangrove rhizosphere was carried out based on their ability to inhibit Vibrio harveyi bacteria that cause vibriosis using the well diffusion method. Determination of inhibitory activity was carried out based on the formation of a clear zone around the well containing the test bacterial culture. A total of 12 bacteria had antibacterial activity from 32 bacteria isolated from the mangrove rhizosphere. The bacterial isolate Sn 1.1 was the bacterial isolate that had the highest antibacterial activity among the 12 bacterial isolates with an inhibition zone diameter of 18.43 mm. Thus, the isolate Sn 1.1 is a potential isolate to be used as a probiotic to control vibriosis in tiger shrimp.

Kata kunci

• Probiotic
• Tiger Shrimp
• Vibriosis
• antibiotics