Utilization of Brazilian Spinach (Alternanthera sissoo) Leaf Extract as a Burn Medicine in Mice (Mus musculus L.)

Abstrak

Brazilian spinach (Alternanthera sissoo hort) leaves contained phytochemical compounds that offer a natural alternative to commercial burn treatments be. This study aimed to evaluate the burn-healing potential of Brazilian spinach leaf extract formulated into an ointment. The leaves were extracted using maceration with 96% ethanol. Phytochemical screening revealed the presence of alkaloids, flavonoids, tannins, saponins, steroids, and vitamin E. The ethanol extract was incorporated into ointments at concentrations of 2%, 4%, and 8%. An in vivo study was conducted using 20 BALB/C mice divided into five groups: negative control (KN), positive control (KP), and three treatment groups (P1: 2%, P2: 4%, P3: 8% extract ointment). Burn injuries were induced by applying heated metal to the shaved dorsal region of the mice. Treatments were applied daily for 7 days. Healing progress was assessed based on burn severity score and burn surface area. Results demonstrated a significant reduction in both parameters across all treatment groups, with the 4% extract ointment (P2) showing the most optimal healing effect. These findings suggest that Brazilian spinach leaf extract, particularly at a 4% concentration, has promising potential as a natural topical agent for burn treatment

Kata kunci

• Alternanthera sissoo hort
• Burn
• Mice
• Surface Area Scoring
• leaves extract