Uji Toksisitas Ekstrak Etanol Daun Jarak Pagar (Jatropha curcas L.) dengan Metode Brine Shrimp Lethality Test (BSLT)

Abstrak

Jatropha curcas plant particularly its leaves, contains secondary metabolites such as flavonoids, alkaloids, tannins, saponins, and phenols. This research aims to determine the toxic activity of ethanol extract of Jatropha curcas leaves on the mortality of Artemia salina larvae, as well as to determine the Lethal Concentration 50 (LC50) value. This study employed six treatment concentrations: 0 ppm, 50 ppm, 100 ppm, 150 ppm, 200 ppm, 250 ppm with 4 repetitions. The Brine Shrimp Lethality Test (BSLT) was used, with 48-hour-old A.salina larvae as the test organism. The results of this study obtained a Lethal Concentration 50 (LC50) value of the ethanol extract of Jatropha leaves of 72.25 ppm. This results indicate the presence of toxic activity against A.salina larvae. Based on the results of the study, the ethanol extract of Jatropha leaves is toxic to shrimp larvae (A.salina) and has the potential as an anticancer compound.

Kata kunci

• Artemia salina
• BSLT
• Jatropha curcas L
• LC50