Uji Antagonis dan Sinergis Antara Isolat Bakteri dan Jamur dari Sampel Batubara Muda (Lignite)

Abstrak

Lignite represents a unique habitat harboring diverse microorganisms with potential application as biological agents. This study aimed to analyze antagonistic and synergistic interactions between bacterial and fungal isolates derived from lignite and to classify their interaction patterns based on multiple inhibition parameters. Interaction assays were conducted using a dual culture method involving 18 bacterial isolates and 5 fungal isolates, resulting in 90 interaction combinations. Observed parameters included inhibition capacity of fungal growth, percentage of inhibition, Percentage of Inhibition Index (PII), and Overall Antagonism Index (OAI). The results demonstrated that all bacterial–fungal combinations exhibited antagonistic interactions against fungal growth, while no synergistic interactions were observed. The degree of antagonism varied among combinations, as reflected by differences in inhibition categories and values of inhibition-related indices. These variations indicate that bacterial–fungal interactions are not uniform and are influenced by the characteristics of each isolate. Overall, the findings of this study indicate the preliminary potential of microorganisms isolated from lignite to be further investigated as candidate biological agents, and they may also be considered for subsequent studies, including the development of microbial consortia to support soil fertility.

Kata kunci

• Bacteria
• Biocontrol
• Fungi
• Lignite
• Microorganisms antagonism