MOLECULAR PHYLOGENY OF BABYROUSA CELEBENSIS CONSTRUCTED FROM MITOCHONDRIAL CYTOCHROME b GENE SEQUENCE

Abstrak

ABSTRACTThe genomic DNA extracted from dried blood of B. celebensis was amplified by using primer pair of DTF2and DTR2 produced a single DNA fragment of 1,140 bp as expected. Sequencing of this fragment confirmedthat the fragment was a fragment of mitochondrial DNA of Cytochrome b gene (Cyt b). Total fragment sizeof complete Cyt b gene of B. celebensis is 1,041 bp with no insertions or deletions (indels) in the sequenceand the translated proteins are 379 amino acids. Nucleotide sequence of Cyt b gene of B. celebensis wasaligned with 15 Cyt b genes (Suidae) deposited in GenBank to contruct phylogenetic tree. The geneticanalysis showed three main clusters, one cluster comprised genus Sus, genus Phacochoerus and anothercluster comprised genus Babyrousa with an outgroup of Bubalus depressicornis that is clearly separated.The sequence similarity (Identity) between B. celebensis and B. babyrussa ranged from 95.2% to 96.4%.Complete sequence of mitochondrial Cytochrome b gene proved to be practical for identification of aBabyrousa species unambiguously.Keywords: DNA extraction, cloning, sequencing, Babyrousa celebensis, Cytochromeb gene and Phylogenetic tree